ABSTRACT
Objective@#The expression patterns of ribosomal large subunit protein 23a (RPL23a) in mouse testes and GC-1 cells were analyzed to investigate the potential relationship between RPL23a expression and spermatogonia apoptosis upon exposure to X-ray.@*Methods@#Male mice and GC-1 cells were irradiated with X-ray, terminal dUTP nick end-labelling (TUNEL) was performed to detect apoptotic spermatogonia @*Results@#Ionizing radiation (IR) increased spermatogonia apoptosis, the expression of RPL11, MDM2 and p53, and decreased RPL23a expression in mice spermatogonia @*Conclusion@#These results suggested that IR reduced RPL23a expression, leading to weakened the RPL23a-RPL11 interactions, which may have activated p53, resulting in spermatogonia apoptosis. These results provide insights into environmental and clinical risks of radiotherapy following exposure to IR in male fertility. The graphical abstract was available in the web of www.besjournal.com.
Subject(s)
Animals , Male , Mice , Apoptosis/genetics , Gene Expression Regulation , Ribosomal Proteins/metabolism , Signal Transduction , Spermatogonia/radiation effectsABSTRACT
OBJECTIVE: To select the optimum extracting procedure for Kangdu Mixture by the application of L9 (34). METHODS: Orthogonal design was used to observe and study the effects of the factors of water volume, extracting times and extracting time on kangdu Mixture, the chlorogenic Acid and yield of dry extract was determined by high performance liquid chromatography, the synthetic weighted mark method was used to evaluate the extraction process. RESULTS: The optimum water extraction is as follows; 10 times of water, extracting twice by relux, 2 hours for each time. CONCLUSION: The process choosed in this experiment is practicable and can be applied for preparation of Kangdu Mixture, the optimal process is simple and high precision.